In order to assess the biological function of proteins and their modifications for understanding signaling mechanisms within cells as well as specific biomarkers to disease, it is important to study the change of proteome under different experimental conditions. The emerging technology of mass spectrometry-based quantitative proteomics provides a powerful tool to quantitatively and systematically assess quantitative differences in protein profiles of distinct samples and is increasingly becoming a significant component of biomedical and clinical research.
Discovery-based quantitative proteomics compares the proteome of a diseased sample versus normal at a global scale, and has been widely used to study various human diseases with the goal to identify biomarkers and/or reveal the pathogenesis of diseases. Methods for acquiring quantitative proteomics data are continually developing with very accurate stable isotope labeling (SIL) and label-free approaches.
SIL provides chemically equivalent but isotopically different interior standards for each peptide/protein for direct comparison of mass spectral signal intensities that represent relative abundance Common SIL strategies include protein and peptide level labeling strategies such as:
Mass spectrometry-based approaches for targeted protein quantification are based on the concept of isotope dilution mass spectrometry techniques commonly used for the detection of small molecules. The approach is comparable to modern advanced antibody-based methods, such as the multianalyte profiling platform (MAP), offering multiplexing detection capability with an excellent dynamic range.
With years’ experience in advanced experiment equipment, Creative Proteomics can provide a variety of proteomics services to assist your scientific research, including:
As one of the leading omics industry company, Creative Proteomics now is opening to provide a series of protein quantification services for our customers. Our service guarantees accurate and reliable results, at quick turnaround time. Please feel free to contact us and see how we can help to address your problems.
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