Visualization of metabolic dynamics is important for all types of metabolic studies, including studies on optimization of biological production processes and studies of metabolism-related diseases. Scientists therefore have used PCA score plots to visualize the metabolic distances, and the PCA score plots for this dataset represent a metabolic map that can be used to study the metabolic flow of pathways and organisms for analysis. Principal component analysis is one of the most frequently applied statistical methods in systems biology, and it is used to reduce the dimensionality of the data while retaining most of the variation in the dataset. This reduction is accomplished by identifying linear combinations of variables, called the principal components, that maximally explain the variation in the data. By comparing these principal components to other features, each sample can be represented by a relatively small number of variables. Nowadays, PCA has been widely applied in transcriptomics and fluxomics, where principal components identify linear combinations of gene or enzyme reactions whose variation in activity explains the largest fraction of variability in the set of samples analyzed. Creative Proteomics has developed a new method for analyzing metabolic turnover analysis data by using PCA strategy.

The main goals of PCA

Principal component analysis (PCA) of metabolic profiles of DT and DS under control and drought stress. Figure 1. Principal component analysis (PCA) of metabolic profiles of DT and DS under control and drought stress. (You, J.; et al. 2019)

Our Services

Creative Proteomics has developed a novel metabolic flux analysis platform to provide principal component analysis (PCA) service in a competitive fashion. We can offer a wide range of services to support all research and development activities.

Our PCA Methodology

We extract the metabolite ion peaks, and normalize the peaks obtained from multiple experimental samples and QC samples. Then, our expert teams perform a comprehensive analysis via PCA. Here are the details of our analysis process:

Principal component analysis (PCA) of a metabolic capacities.  Figure 2. Principal component analysis (PCA) of a metabolic capacities. (Nobu, M, K.; et al. 2020)

Features of Our MFA Platform

Based on high-performance quantitative techniques and advanced equipment, Creative Proteomics has constantly updating our metabolic flux analysis platform and is committed to offering professional, rapid and high-quality services of principal component analysis (PCA) at competitive prices for global customers. Our personalized and comprehensive services can satisfy any innovative scientific study demands, please contact our specialists to discuss your specific needs. We are looking forward to cooperating with you!

References

  1. You, J.; et al. Transcriptomic and metabolomic profiling of drought-tolerant and susceptible sesame genotypes in response to drought stress. BMC Plant Biology. 2019. 19(1).
  2. Nobu, M, K.; et al. Catabolism and interactions of uncultured organisms shaped by eco-thermodynamics in methanogenic bioprocesses. Microbiome. 2020. 8: 111.

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