Metabolomics can reflect static metabolite abundance, however, the increases in a single metabolite may be due to activation of synthetic pathways or inhibition of the consumption pathways. Therefore, metabolomics is often insufficient to solve all the issues in the study of specific metabolic pathways and metabolic networks. As a complementary tool to metabolomics, metabolic flux analysis is a powerful tool capable of quantifying energy flow (i.e., ATP, cofactor production and consumption) and their balance. Flux variations for a particular metabolic pathway are not necessarily directly related to metabolite concentrations measured at specific time points within that pathway. However, metabolic flux and metabolic pathway kinetics can be defined via targeting mass spectrometry measurements of substrates and isotopes at continuous time points. In addition to the stable isotope 13C, Creative Proteomics also supports the use of 15N to label specific molecules and track their metabolic processes in the organism to obtain information on the dynamics of metabolites in metabolic pathways.

Summary of current 13C-MFA studies on different organisms. Figure 1. Summary of current 13C-MFA studies on different organisms. (Sasnow, S. S.; et al. 2015)

Our Services

Creative Proteomics has developed a novel metabolic flux analysis platform to provide 15N-MFA service in a competitive fashion. We can offer a wide range of services to support all research and development activities.

15N-MFA Work Flow

Application of Our Services

Features of Our MFA Platform

Based on high-performance quantitative techniques and advanced equipment, Creative Proteomics has constantly updating our metabolic flux analysis platform and is committed to offering professional, rapid and high-quality services of 15N-MFA at competitive prices for global customers. Our personalized and comprehensive services can satisfy any innovative scientific study demands, please contact our specialists to discuss your specific needs. We are looking forward to cooperating with you!

Reference

  1. Sasnow, S. S.; et al. Bypasses in intracellular glucose metabolism in iron-limited Pseudomonas putida. MicrobiologyOpen. 2015.

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