Cellular motility is vital in many physiological and pathological processes and this motility is also a response to extracellular stimuli, which is the fundamental to numerous biological processes such as inflammation, wound healing and so on. Abundant studies centered on the identification and characterization of factors that regulate and direct cell movement. Many of these experiments have shown that the cell migration can be interfered with the host serum components and extracellular matrix breakdown products. The basement membrane and extracellular matrix components can also promote cellular haptotaxis, which can influence the results of cell migration. The method that use colloidal particle coated surface is a simple way to measure migration on the signal cell level and this approach can avoid many other distractions and makes it possible to generate better results for the single cell migration assay.
The method mentioned above, which is to use colloidal gold particle coated surface for the single cell mobility assay, is also called colloidal gold single cell migration assay. This approach to research single cell migration is very convenient and simple. There are precast tissue culture plates coated with colloidal gold particles and cells are seeded onto these plates at low density. The gold colloidal particles are seen as a homogenous layer of small dark dots under the microscope and the gold particles can be phagocytized by the migrating single cells. When cells migrate from one to another place, there is a white track to record the cell migration. That’s because the gold colloidal particles in the trail are phagocytized by the migrating cells and removed from the plastic surface. The cleared areas can be evaluated quantitatively and the single cell migration can be characterized by this method. This single cell mobility assay is used to track cell migratory paths and it could also be used to demonstrate the motility of tumor cells at the single cell level.
The colloidal gold single cell migration assay can make single cell tracking possible and the undirected movement also can be monitored. This assay has many other advantages such as the real-time path of cell migration can be obtained and the absolute speed of migration can be determined. The automation and high throughput living image system and analysis system in our laboratory also ensure better results of cell migration up to your requirement.
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