HPLC Analysis Service
High Performance liquid chromatography (HPLC) is a technique able to separate, identify, and quantify each component in a mixture. The sample mixture contained in liquid solvent is pressurized to pass through a column filled with a solid adsorbent material. Each component in the mixture interacts differently between stationary phase and mobile phase, causing different components with different flow rates and leading to the components separation as they flow out the column. Thus different proteins in complex mixture can be separated through PHLC according to their unique retention time. Peak area integral can be used to detect the protein purity.
As system pressures (0–400 bar) are significantly higher, HPLC is distinguished from traditional liquid chromatography. HPLC columns usually are made with smaller sorbent particles, causing superior resolving power (the ability to distinguish between compounds) when separating mixtures. In conclusion, it will cost less time and improve sensitivity for analysis through HPLC, which makes it a popular chromatographic technique.
Different separation mechanisms include adsorption, distribution, ion exchange, size exclusion, hydrophobic interaction and affinity. Majority of organic compounds can be separated and analyzed through HPLC.
Types of chromatography techniques
- Size exclusion Chromatography (SEC);
- Reversed-phase Chromatography (RP);
- Normal-phase Chromatography;
- Ion-exchange Chromatography;
- Affinity Chromatography;
- Hydrophobic Interaction Chromatography (HILIC);
- Ion-pairing Chromatography;
Pronalyse offers various chromatography techniques for purification analysis of biopharmaceuticals. Contact Us for Detailed Informations!!