Stable isotope labeling by amino acids in cell culture (SILAC) is a popular and mass spectrometry-based technique for quantitative proteomics. SILAC mouse is a novel in vivo proteomics approach that allows the quantitative determination of protein patterns of mouse tissues. The principle of SILAC mouse is similar to that of SILAC approach aiming at the quantification of proteins in cell cultures. The mouse is almost labeled by feeding the heavy diet. SILAC mouse has various applications. The SILAC mouse can serve as a reference model at any biological scale, including single proteins of interest and the whole organ. In addition, some cell types like intestinal epithelium that are difficult to analysis ex vivo can be studied by the in vivo SILAC mouse.

Figure 1. Mice are SILAC labeled by being fed a pelleted, lysine-free diet supplemented with normal or heavy lysine. (Krüger M, et al., 2008)

As a supplier of isotope labeled products in the industry, Creative Proteomics offers more than 10,000 products labeled with different isotopes of carbon, hydrogen, nitrogen, and oxygen. Creative Proteomics supplies SILAC mouse feed, including Mouse Express^® unlabeled mouse feed, MouseExpress^® L-leucine mouse feed kit, MouseExpress^® L-lysine mouse feed kit, spirulina mouse feed, and so on. Custom synthesis is also available to meet our customers’ research needs.

Reference:

1.Krüger M, et al. SILAC mouse for quantitative proteomics uncovers kindlin-3 as an essential factor for red blood cell function. Cell, 2008, 134(2): 353-364.