Proteins or peptides are easily modified through chemical synthesis at primary amines or cysteine sulfhydryl groups using chemical tagging reagents. Tagging reagents are compatible with almost any type of biological sample type and represent a low-cost alternative to metabolic labeling. One popular and simple chemical tagging methods is dimethylation. Reductive methylation utilises formaldehyde and cyanoborohydride or cyanoborodeuteride and results in the addition of two methyl groups on the N-terminus and lysine side chains. Dimethylation also allows for the quantification of two or three biological samples in mass spectrometry experiment.

As a supplier of isotope labeled products in the industry, Creative Proteomics offers more than 10,000 products labeled with different isotopes of carbon, hydrogen, nitrogen and oxygen. Creative Proteomics supplies stable isotope labeled chemical tagging reagents such as Acetaldehyde-D4, Bromobenzene-13C6, Iodoacetamide-D4, Sodium acetate-2-13C and Propionic acid-D6. Custom synthesis of isotope labeled chemical tagging reagents is also available to meet our customers’ needs.