NAGPA Full-Length MS Protein Standard


Product Description:

Name NAGPA Full-Length MS Protein Standard
Cat# CPFL318271
Protein NameN-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
SymbolNAGPA
SynonymsAPAA; UCE
AccessionNM_016256
Cytogenetic16p13.3
Amino Acid Labeled[U- 13C6, 15N4]-L-Arg and [U- 13C6, 15N2]-L-Lys
Chemical Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
Expression HostHuman HEK293 cells
Predicted MW53.3 kDa
Expression TrueORF CloneRC218271
DescriptionNAGPA Full-Length MS Protein Standard (NP_057340), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as uncovering enzyme or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. This reaction most likely occurs in the trans-Golgi network. This enzyme functions as a homotetramer of two disulfide-linked homodimers. In addition to having an N-terminal signal peptide, the proteins C-terminus contains multiple signals for trafficking it between lysosomes, the plasma membrane, and trans-Golgi network.

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