Transwell Invasion Assay

Cell invasion assay plays an important role in the studies of cell behaviors and assessment of the metastatic potential of tumor cells. Among the various cell invasion assays, transwell invasion assay is easily used and widely applied, such as human trophoblast, melanoma and colon cancer invasion. In transwell invasion assay, two medium containing chambers separated by a porous membrane. The porous filter is blocked by a gel composed of the extracellular matrix (ECM) that is meant to mimic the typical matrices during the invasion process in vivo to block the pore in the membrane. The ECM can occlude the membrane pores and invasive cells can degrade the matrix and move through the ECM layer, whereas the non-invasive cells will be blocked from migrating through the ECM layer. The cells are placed on one side of the gel and a chemoattractant is placed on the other side of the gel. We can determine the invasion by counting the cells that have traversed the cell-permeable membrane having invaded towards the higher concentration of chemoattractant.

Overview of Transwell Invasion Assay Service

At Creative Proteomics, we have much experience in providing a complete transwell assay for the invasion in various formats and extracellular matrices to suit your research needs. Our process for transwell invasion assay includes:

  • Preparation including cell maintenance and transwell coating.
  • Plating cells. Cell suspension is placed in the upper chamber and incubation for 4 to 24 hours
  • Staining and counting cells. After removing non-invasive cells, invaded cells are stained and quantified.
  • Data interpretation.

Transwell-invasion-assay

Sample Requirement

The cell lines for assays should be provided by customers and our company can provide commonly used cell lines. If you want to know specific samples requirements, please feel free to contact us.

Deliverable

We can provide a detailed report including experimental process, raw data, pictures, and analysis results. In addition, we can provide other reports according to customers’ requirements.

Advantages

  • Different cell types are available, including cancer cell lines, endothelial cells, epithelial cell astrocytes, leukocytes, lymphocytes, monocytes, etc.
  • We can design experiments according to customers’ needs.
  • Different pore sizes are suitable for different cell types, including 12 µm, 8 µm, 5 µm, 3 µm.
  • 6-well, 12-well, 24-well, and 96-well formats are available for different assays.

Transwell invasion assay has many advantages in the study of cell invasion, such as relatively easy to set up and cost-effective. At Creative Proteomics, based on advanced platforms including the high throughput living imaging system, our excellent and experienced experts will optimize the experimental protocol according to your requirement and provide transwell invasion assay to guarantee the high-quality results for your research. As every project has different requirements, please contact our specialists to discuss your specific needs.

Reference
1. Marshall J. Transwell® invasion assays. Cell Migration. Humana Press, 2011: 97-110.


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Tel: 1-631-275-3058

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Stay in Contact

USA

  • 45-1 Ramsey Road, Shirley, NY 11967, USA
  • Tel: 1-631-275-3058
  • Fax: 1-631-614-7828
  • Email:

Europe

  • Tel: 44-208-144-6005
  • Email:
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