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Determination of Amino Acid Composition


Amino acids are the building blocks of proteins, and are the fundamental nutrients for human beings. There are 20 amino acids for the composition of proteins. 10 out these amino acids can be synthesized by humans, and are known to be nonessential amino acids. Whereas, the rest cannot be produced by humans, and must be supplied in the food, hence, are known as essential amino acids. Composition and structure of these 20 amino acids are different, so as the their perspective functions.

Besides acting as nutrients, recent findings revealed that amino acids also serve as an important activator for certain signaling pathway. The well-known mTOR signaling pathway has been proven to sense the level of amino acid. Presence of certain amino acids activates the mTOR pathway, whereas deprivation of amino acid inhibits this pathway. Another example is that GCN2 (general amino acid control non-derepressible 2) senses one or more amino acids by virtue of direct binding to uncharged cognate tRNAs. These studies in all show amino acids play a very crucial role in regulating the physiological functions, and its potential function is far beyond acting as nutrients only.

Determination of Amino Acid Composition

Determination of Amino Acid Composition

This service analyzes both relative amino acid composition and free amino acids. The relative amino acid composition gives a characteristic profile for proteins, which is often sufficient for identification of a protein. It is often used as decision support for choice of proteases for protein fragmentation.

Currently there are 3 ways for amino acid analysis: HPLC, automatic amino acid analyzer and LC-MS/MS. In the most experiments, the analytical procedures usually include hydrolysis of protein/peptides in the samples, derivatization of free amino acids pre/post column, separation and quantitation of the amino acids. Not only the 20 natural amino acids, but also all the known amino acids which may exist in the biological samples, such as γ-aminobutyric acid (GABA), L-Ornithine, can be identified and quantified with proper internal standards and calibration curves.

Free amino acids determination service:

Analysis of 20 natural amino acids, all the known amino acids which may exist in the biological samples, such as γ-aminobutyric acid (GABA), L-Ornithine, etc.

Workflow of analysis of free amino acids:

  • Removal of protein by precipitation
  • Analyze amino acid in ion exchange chromatography and MS
  • Data analysis and quantification of amino acids

Workflow of protein amino acid analysis:

  • Protein hydrolysis in acidic condition, with 6 M hydrochloric acid (24 hours, 110°C) to break down proteins into amino acids
  • Separation of amino acids through HPLC
  • Analyze amino acids using aromatic amino acid analyzer
  • Data analysis and quantification of 20 amino acids

Feature and Advantage of amino acid composition analysis service:

  • Cutting-edge facilities
  • Reliable & Reproducible results
  • Highly sensitive: detect low abundance amino acids
  • Comprehensive analysis of amino acids composition

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