 We have established a protein separation system that employs affinity chromatography, ion exchange, hydrophobic chromatography and gel filtration to separate proteins. Notably, our PF2D protein fractionation system can perform intact protein separation and quantitation simultaneously. This system is especially suitable for comparative profiling of crude cell lysates, sera, and other samples; also it has the ability to separate each sample into ~500 fractions.
In contrast, separation of peptides is typically completed by reverse phase HPLC. We have also established a tandem LC system to improve the peptide separation.
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