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HCV Protease FRET Substrate MS Grade peptide

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Product Description


NameHCV Protease FRET Substrate MS Grade peptide
Cat#CPWHB105219
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DescriptionA HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em=355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.
Chemical FormulaC68H89N15O25S
Molecular Weight1548.6 Da
SequenceAc-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-EPI-(COO)Ala-Ser-Lys(DABCYL)-NH2

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