Home > Products > Mass Spectrometry Products > MS Peptide Standards > HCV Protease FRET Substrate MS Grade peptide

HCV Protease FRET Substrate MS Grade peptide


Product Description

NameHCV Protease FRET Substrate MS Grade peptide
Molecular Weight1548.6 Da
Chemical FormulaC68H89N15O25S
DescriptionA HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em=355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.

Online Inquiry

Please input "proteomics" as verification code.